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Raji cell
Raji cell line is the first continuous human cell line from hematopoietic origin. The cell lines produce an unusual strain of Epstein-Barr virus which will both transform cord blood lymphocytes and induce early antigens in Raji cells. Translocations between chromosomes 8 and 22 have occurred in all three lines, but the cells synthesize immunoglobulin M with light chains of the kappa type, in contrast to the usual concordance between a translocation involving chromosome 22 and lambda chain synthesis. Both kappa genes and one lambda gene are rearranged. These findings indicate either that translocation may occur as a separate event from immunoglobulin gene rearrangement or that the proposed hierarchical sequence of immunoglobulin gene rearrangements is not always adhered to. The data also imply that in cells containing a translocation between the long arm of chromosome 8 and a chromosome bearing an immunoglobulin gene, alteration of cellular myc expression may occur regardless of the immunoglobulin gene that is expressed.〔http://www.sciencemag.org/gca?sendit.y=9&gca=sci%3B222%2F4628%2F1094&sendit=Get%20all%20checked%20abstract%28s%29〕 Raji cell is widely used as a transfection host and also to understand the hematopoietic and other cell malignancies. It also use for detection of immune complex because it possesses and expresses and lots of receptors for certain complement components, as well as Fc receptors for immunoglobulin G.
==Background==
Based on the morphological characteristic, this cell is categorized as lymphoblast-like.〔(【引用サイトリンク】title=ATCC-86: Raji Cell )〕 This suspension cell is derived from B-lymphocyte of an 11-year-old Nigerian Burkitt's lymphoma male patient in 1963. R.J.V. Pulvertaft is the first person who established this cell line.〔 The culture medium used to grow this cell line is RPMI supplemented with serum. Some characteristics of Raji cell is lack of differentiation thus shows the formation of large aggregations consist of hundreds individual cells. The cells are relatively large in diameter (5-8 μm), have irregular indented nuclei, and almost extensive cytoplasm with free ribosomes which tend to clump.〔(【引用サイトリンク】title=JCRB9012 () )〕 Raji cell grows as single, non-motile, free-floating (non-adhesion) individuals or doublets to glass. Some cells look elongated like pear-shaped with larger, multinucleate, round cells.〔
Raji cell is widely used as a transfection host and also to understand the hematopoietic and other cell malignancies. It also use for detection of immune complex because it possesses and expresses and lots of receptors for certain complement components, as well as Fc receptors for immunoglobulin G.〔
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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